PGP9.5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91066-50, BS91066-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09936(Human) Q9R0P9(Mouse) Q00981(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
IP:1:50-1:100

Background: UCH-L1 (ubiquitin C-terminal hydrolase) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. UCH-L1 is expressed in brain neurons. Examination of specific brain regions reveals expression in all areas tested, particularly in the substantia nigra. UCH-L1 represents 1-2% of total soluble brain protein. Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease. The gene which encodes UCH-L1 maps to human chromosome 4p13. The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1. UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PGP9.5 polyclonal antibody detects endogenous levels of PGP9.5 protein.

Bioworld Molecular Weight: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PGP9.5 on A549 cell lysates using anti-PGP9.5 antibody at 1/1,000 dilution., ICC staining PGP9.5 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: UCH-L1, PGP9.5, UCHL1, Epididymis luminal protein 117, Epididymis secretory protein Li 53, HEL 117, HEL S 53, NDGOA, Neuron cytoplasmic protein 9.5, OTTHUMP00000218137, OTTHUMP00000218139, OTTHUMP00000218140, OTTHUMP00000218141, Park 5, PARK5, PGP 9.5, PGP9.5, PGP95, Protein gene product 9.5, Ubiquitin C terminal esterase L1, Ubiquitin C terminal hydrolase, Ubiquitin C terminal hydrolase L1, Ubiquitin carboxyl terminal esterase L1, Ubiquitin carboxyl terminal hydrolase isozyme L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, UCH-L1, UCHL1, UCHL1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified